Mussel-like adhesive protein with strong adhesive force and preparation method thereof

The invention discloses a mussel-like adhesive protein with strong adhesive power and a preparation method thereof, and belongs to the field of adhesive proteins. The pI of each of the mussel-like mucoprotein intermediates mlp353, mlp316 and mlp319 provided by the invention is about 10, and the mussel-like mucoprotein intermediates contain tyrosine (Tyr) with the mole percent of 23.1%-25.5%. The mussel-like adhesive protein intermediate is oxidized by tyrosinase to obtain new mussel-like adhesive protein, and compared with natural mussel adhesive protein, the mussel-like adhesive protein has high adhesive power and uniform molecular weight distribution, can be used for preparing an adhesive material with high adhesive power, and can be used for preparing the mussel-like adhesive protein. Wide application prospects are realized in the fields of ocean engineering, biomedicine, surface chemistry and the like. 本发明公开了一种粘附力强的类贻贝粘蛋白及其制备方法，属于粘附蛋白领域。本发明提供的类贻贝粘蛋白中间体mlp353，mlp316及mlp319的pI均在10左右，且含有高达23.1％～25.5％摩尔百分数的酪氨