Preparation method of autologous fat collagen injection

The invention discloses a preparation method of an autologous fat collagen injection. The preparation method comprises the following steps: S1, culturing autologous fat at constant temperature; s2, cutting and scattering the autologous fat treated in the step S1, and centrifugally removing oil; s3, blowing and suspending the fibroblasts in the S2, and culturing at constant temperature to enable the fibroblasts to secrete collagen; s4, centrifuging the venous blood sample in a test tube containing an anticoagulant, and centrifuging the supernate to obtain plasma rich in platelets and growth factors; and S5, uniformly mixing fibroblasts, collagen and the plasma in the step S4 to obtain the autologous fat collagen injection. According to the method disclosed by the invention, 90% of the oil-free drops in the autologous fat are removed, and the components with poor supporting property and low survivability are continuously removed in combination with a centrifugal technology, so that the collagen with good suppor