Compositions derived from human amniotic cells and related methods

Disclosed is a method of preparing an acellular human amnion derived composition configured for therapeutic use, the method generally comprising the steps of obtaining amnion tissue; detecting pathogens of the amniotic tissue; cleaning the amniotic tissue; manually removing blood-containing chorionic tissue from the amniotic tissue, and decellularizing the amniotic tissue with a non-heterogeneous enzyme; collecting amniotic cells from the decellularized amniotic tissue; inoculating amniotic cells for culture into a non-heterogeneity culture medium prepared for the mesenchymal stem cells; enabling the amniotic cells to grow to a specific confluence degree; collecting a conditioned medium; freezing the collected conditioned medium; wherein the method further comprises irradiating the conditioned medium. 披露了一种制备被配置用于治疗用途的无细胞人羊膜衍生组合物的方法，该方法一般包括以下步骤：获得羊膜组织；检测该羊膜组织的病原体；清洗该羊膜组织；从该羊膜组织手动去除含血绒毛膜组织，用无异种酶使该羊膜组织脱细胞；从经脱细胞的羊膜组织收集羊膜细胞；将用于培养的羊膜细胞接种到针对间充质干细胞配制的无异种培养基中；使这些羊膜细胞生长到特定的汇合度；收集条件培养基；并冷冻收集的条件培养基；其中该方法进一步包括照射该条件培养基。