Cell culture medium based on porous gel array and preparation method thereof

The invention discloses a preparation method of a cell culture medium based on a porous gel array, which comprises the following steps: preparing a sodium hyaluronate solution, dropwise adding methacrylic anhydride for the first time in an ice-water bath, reacting, dropwise adding methacrylic anhydr...

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1. Verfasser: ZHUGE XIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a preparation method of a cell culture medium based on a porous gel array, which comprises the following steps: preparing a sodium hyaluronate solution, dropwise adding methacrylic anhydride for the first time in an ice-water bath, reacting, dropwise adding methacrylic anhydride for the second time, continuously reacting, adding excessive glacial ethanol after the reaction is finished, and centrifuging to obtain the cell culture medium based on the porous gel array. Collecting the precipitate, dissolving the precipitate in the deionized water again, and then freeze-drying to prepare the methacrylic acid esterified sodium hyaluronate; mixing and stirring the prepared methacrylic acid esterified sodium hyaluronate, the reconstructed RGD adhesion peptide and 0.01 MOL/L phosphate buffer solution, then adding dithiothreitol, and uniformly mixing, so as to prepare a gel stock solution; and by taking polydimethylsiloxane filled with microcolumns as a template, carrying out casting molding on