6-benzyladenine hapten, artificial antigen and antibody as well as preparation method and application of 6-benzyladenine hapten and artificial antigen

The invention provides a 6-benzyladenine hapten, an artificial antigen, an antibody as well as preparation and application of the 6-benzyladenine hapten, the 6-benzyladenine artificial antigen and the antibody, the 6-benzyladenine hapten 6-BA-2C is prepared, the hapten 6-BA-2C is coupled with carrier protein BSA to obtain the artificial antigen 6-BA-2C-BSA, and the specific antibody for detecting 6-benzyladenine is further prepared. An artificial antigen 6-BA-2C-OVA is used as a coating antigen, the antibody has good sensitivity and specificity to 6-benzyladenine, the semi-inhibitory concentration is 1.18 [mu] g/Kg, the minimum detection limit is 0.056 [mu] g/Kg, the quantitative detection range is 0.17-61.04 [mu] g/Kg, and the antibody has no cross reaction to zeatin, trans-zeatin and N6-benzoyl adenine, and can be used for detecting 6-benzyladenine. The 6-benzyladenine immunoassay method with higher specificity and sensitivity is established by using the antibody disclosed by the invention. 本发明提供了一种6-苯甲基腺嘌呤