Preparation method of semi-solid armillaria mellea strain

The invention provides a preparation method of a semi-solid armillaria mellea strain, which is characterized in that an armillaria mellea stock culture medium is prepared into a'jelly 'semi-solid state and contains solid and liquid, so that the germination speed, the growth speed and the p...

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Bibliographische Detailangaben
Hauptverfasser: ZHANG XIAOPING, WANG SHAOBAI, NIE TIANXIA, ZHANG SHENGMING, GONG WEI, GONG ANQING, XU CHENG, GONG ANSHENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a preparation method of a semi-solid armillaria mellea strain, which is characterized in that an armillaria mellea stock culture medium is prepared into a'jelly 'semi-solid state and contains solid and liquid, so that the germination speed, the growth speed and the purity (yield) of an armillaria mellea stock strain on the stock culture medium are remarkably improved; meanwhile, when the armillaria mellea cultivar is prepared, a sealing mulch culture medium is prepared, so that the germination speed and the growth speed of stock seeds on the cultivar culture medium are obviously increased, the stock seeds are quickly sealed, infectious microbe pollution is reduced, and the yield of the cultivar is increased by 5-8%; the hypha growth temperature is regulated and controlled in a segmented manner when the armillaria mellea cultivar is cultured, namely, the earlier-stage culture temperature is 15-18 DEG C, the middle-later-stage culture temperature is 22-24 DEG C, and the preservation tempe