Method for reducing mutation rate of long fragment sequence synthesis

The invention provides a method for reducing the mutation rate of long fragment sequence synthesis. The method comprises the following steps: (1) obtaining oligonucleotide primer sequences with uniform length; (2) synthesizing by using a synthesizer; (3) separating the synthesized oligonucleotide primer from the solid-phase carrier; (4) mixing the obtained primers with the same length, recovering and purifying; the purified oligonucleotide primer is subjected to enzyme digestion, and redundant T/N basic groups are cut off; and (5) carrying out Overlap PCR (Polymerase Chain Reaction) to synthesize a long fragment sequence. According to the method disclosed by the invention, all primers with the same length can be obtained only through one-time gel recovery and enzyme digestion, the error rate of primer synthesis is reduced, the error rate of long fragment synthesis can be greatly reduced in subsequent long fragment sequence synthesis, the synthesis accuracy is improved, the synthesis cost is reduced, and the s