Kit for detecting CD16A gene polymorphism as well as application and use method thereof

The invention discloses a kit for detecting CD16A gene polymorphism as well as application and a use method of the kit. The kit comprises a first primer pair for amplifying a CD16A gene sequence, a second primer pair for detecting the rs396991 site polymorphism of a CD16A gene and a fluorescent probe, the first primer pair is used for amplifying a CD16A gene sequence; the second primer pair is used for detecting the rs396991 site polymorphism of the CD16A gene; the fluorescent probe comprises a probe for detecting the rs396991 site polymorphism of the CD16A gene, the probe comprises a first probe 158F-probe: 5 '-FAM-TTACTCCAAAAAGCCCCCTG-MGB-3' and a second probe 158V-probe: 5 '-HEX-TTACTCCAAAAAGCCCCCTG-MGB-3', and the fluorescent probe has the advantages of being simple and convenient to operate, high in accuracy, good in specificity, low in cost and the like. 本发明公开了一种检测CD16A基因多态性的试剂盒及其应用和使用方法，试剂盒包括扩增CD16A基因序列的第一引物对和检测CD16A基因rs396991位点多态性的第二引物对和荧光探针；扩增CD16A基因序列的第一引物对；检测CD16A基因rs396991位点多态性的第二引物对；荧光探针包括检测CD16A