AAV8 neutralizing antibody detection method and detection kit

The invention discloses a method for detecting an AAV8 neutralizing antibody, which comprises the following steps: using a proper serum diluent as a sample diluent, co-incubating with a to-be-detected sample by using a gene element AAV8 carrier containing autonomously expressed firefly enzyme, infecting 293T cells with an AAV8 sample mixed solution, and characterizing the neutralizing activity by detecting a chemiluminescence signal. The AAV8 neutralizing antibody detection method provided by the invention is high in sensitivity, controllable in source and applicable to a detection kit for the AAV8 neutralizing antibody in a human blood matrix, AAV8 capable of autonomously expressing firefly enzyme and a human serum sample are incubated, and then 293T cells are infected, so that the AAV8 neutralizing antibody is obtained. After incubation for a period of time, the activity of the neutralizing antibody in a human serum sample is detected by detecting the expression of firefly enzyme of the 293T cell. The metho