Preparation method of kidney cancer antigen CA9 protein specific cytotoxic T lymphocyte

The invention provides a preparation method of kidney cancer antigen CA9 protein specific cytotoxic T lymphocytes, and belongs to the technical field of immune cell therapy. The method disclosed by the invention comprises the following steps: preparing an rAAV2-siSOCS1-CA9 recombinant virus vector carrying target genes of immune negative regulation inhibition genes siSOCS1 and CA9; separating and culturing DC cells in mononuclear cells; carrying out rAAV2-siSOCS1-CA9 recombinant virus vector infection, and inducing the DC cells to be mature, so as to obtain iAPA-CA9-DC cells; and carrying out mixed culture on the iAPA-CA9-DC cells and T cells to obtain the iAPA-CA9-DC/CTL cells with antigen specificity, namely the dendritic cell activated kidney cancer protein CA9 specific cytotoxic T lymphocytes. According to the method disclosed by the invention, a large number of tumor antigen specific T cells are cultured and amplified, so that the aim of treating cancers is fulfilled. 本发明提供了一种肾癌抗原CA9蛋白特异性细胞毒性T淋巴细胞的制备方法，属