Armored RNA (Ribonucleic Acid) standard substance containing novel coronavirus ORF1ab gene, E gene and N gene and application thereof

The invention discloses an armored RNA (Ribonucleic Acid) standard substance containing a novel coronavirus ORF1ab gene, an E gene and an N gene. The standard substance is formed by packaging a novel coronavirus ORF1ab gene, an E gene and an N gene with capsid protein of an MS2 bacteriophage by using an MS2 bacteriophage armored RNA technology, and the prepared standard substance has the characteristics of stability, no biological infectivity, capability of truly simulating a virion structure, RNase resistance and the like, and can be used for preparing the standard substance. The method is suitable for the quality control of a nucleic acid detection method taking the ORF1ab gene, the E gene and the N gene as targets for the new coronavirus, and the evaluation of detection reagents and laboratory ability. 本发明公开了一种内含新型冠状病毒ORF1ab基因、E基因和N基因装甲RNA标准物质。该标准物质是利用MS2噬菌体装甲RNA技术，将新型冠状病毒ORF1ab基因、E基因和N基因用MS2噬菌体的衣壳蛋白包装而成，本发明制备的标准物质具有稳定、无生物传染性、可真实模拟病毒粒子结构、耐RNase等特性，适用于新冠病毒以ORF1ab基因、E基因和N基因为靶标的核酸检测方法的质量控制，以及检测试剂和实验室能力的评价。