Application of high-throughput screening tool for enabling Escherichia coli to obtain effective NHEJ system in Escherichia coli gene editing

The invention provides application of a high-throughput screening tool for enabling escherichia coli to obtain an effective NHEJ system in escherichia coli gene editing. The high-throughput screening tool for enabling the Escherichia coli to obtain the effective NHEJ system comprises a pDual-Cas9-Parental plasmid vector and a high-throughput screening vector, wherein the pDual-Cas9-Parental plasmid vector contains a DNA helicase gene, a replicon, an antibiotic resistance gene, a nuclease gene, an araC gene, an arabinose promoter and an IIs type restriction enzyme recognition site; and the pDual-sgRNA-lacZ plasmid vector contains an sgRNA sequence of a targeted lacZ gene, a strong promoter for constitutive expression, a replicon and an antibiotic resistance gene. The NHEJ system obtained through screening has good connection efficiency in escherichia coli, efficient gene editing can be carried out, and the application prospect is wide. 本发明提供了一种使大肠杆菌获得有效NHEJ系统的高通量筛选工具在大肠杆菌基因编辑中的应用，所述使大肠杆菌获得有效NHEJ系统的高通量筛选工具包括：pD