Hybridoma cell strain secreting anti-alpha-conotoxin monoclonal antibody and application thereof

The invention particularly relates to a hybridoma cell strain 5E4 capable of secreting an anti-alpha-conotoxin monoclonal antibody and application of the hybridoma cell strain 5E4. The hybridoma cell strain is classified and named as an anti-alpha-conotoxin monoclonal antibody hybridoma cell strain,...

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Bibliographische Detailangaben
Hauptverfasser:	WANG RONGZHI, TANG HENGKUN, YANG MINYI, LING SUMEI, WANG SHIHUA, GAO YEHONG, CHEN RUI, LIU HAIMEI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CASTING CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MAKING METALLIC POWDER MANUFACTURE OF ARTICLES FROM METALLIC POWDER MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NANOTECHNOLOGY ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHYSICS POWDER METALLURGY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES SPIRITS TESTING TRANSPORTING VINEGAR WINE WORKING METALLIC POWDER
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Beschreibung
Zusammenfassung:	The invention particularly relates to a hybridoma cell strain 5E4 capable of secreting an anti-alpha-conotoxin monoclonal antibody and application of the hybridoma cell strain 5E4. The hybridoma cell strain is classified and named as an anti-alpha-conotoxin monoclonal antibody hybridoma cell strain, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 24, 2020, and the preservation number is CGMCC No.22347. The anti-conotoxin alpha B-VxXXIVA-CTX monoclonal antibody secreted by the hybridoma cell strain 5E4 is strong in specificity, large in affinity and high in titer, and can be applied to the technical field of conotoxin detection. Based on the hybridoma cell strain 5E4 and the monoclonal antibody secreted by the hybridoma cell strain 5E4, an immune lateral flow chromatography detection card is established, and the detection card can be applied to conotoxin alpha B-VxXXIVA-CTX detection and has the advantages of being high in sensitivity and rapid in detection. 本发明具体