Isolated oncolytic adenoviruses capable of expressing exogenous genes, vectors, therapeutic agents and uses thereof

The present invention provides isolated oncolytic adenoviruses capable of expressing exogenous genes, vectors, therapeutic agents, and uses thereof. The oncolytic adenovirus is a selective replicating type recombinant oncolytic adenovirus obtained by carrying out gene modification on an adenovirus, and the genome of the oncolytic adenovirus has the following characteristics: 1) the oncolytic adenovirus contains an E1B gene regulatory element; (2) an E1B gene coding region is deleted, an exogenous gene is inserted into the site, and the exogenous gene is positioned behind an E1B promoter and is controlled by an E1B gene regulation element; and 3) cDNA (complementary deoxyribonucleic acid) of E1A for transcribing E1A 13s mRNA (messenger Ribonucleic Acid) on the upstream of the exogenous gene, wherein the cDNA is of a wild type or an Rb protein binding region deletion type. The oncolytic adenovirus provided by the invention can more effectively introduce nucleic acid with a labeled polypeptide coding sequence in