Method for simultaneously detecting multiple components in Daqili preparation

The invention belongs to the field of quality detection of traditional Chinese medicines, and particularly relates to a method for simultaneously detecting multiple components in a Daqili preparation, which comprises the following steps: respectively carrying out content determination and result jud...

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Bibliographische Detailangaben
Hauptverfasser: REN QI, ZHANG LIQUN, XU YAN, ZHENG YINGYING, JIANG JUNHUA, WANG HUAN, WU LIANGFA
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention belongs to the field of quality detection of traditional Chinese medicines, and particularly relates to a method for simultaneously detecting multiple components in a Daqili preparation, which comprises the following steps: respectively carrying out content determination and result judgment on a mixed reference solution and a test solution by adopting an HPLC (High Performance Liquid Chromatography) method, and carrying out gradient elution by taking octadecylsilane chemically bonded silica as a chromatographic column filler and acetonitrile as a mobile phase A, a 0.1% formic acid solution is used as a mobile phase B, gradient elution is carried out, and the number of theoretical plates is not lower than 4000 according to rhaponticin peaks; the reference substance solution contains abietic acid, rhaponticin, loureirin B and 11-carbonyl-beta-acetyl masticinic acid. The method capable of simultaneously detecting multiple components in the Daqiqi preparation is established, and the method is good i