Method for measuring concentration of hydrocortisone in human plasma by liquid chromatography-mass spectrometry

The invention belongs to the technical field of medicine detection, and particularly discloses a method for determining the concentration of hydrocortisone in human plasma by liquid chromatography-mass spectrometry, which comprises the following steps: extracting hydrocortisone in plasma by adopting a liquid-liquid extraction method to obtain an extracting solution; the extraction solution is subjected to qualitative and quantitative determination of the concentration of the hydrocortisone in the human plasma by adopting high performance liquid chromatography-tandem mass spectrometry, the problem that the plasma matrix has many interference is solved, the sensitivity is high, the result is accurate and reliable, the matrix effect, the hyperlipidemia matrix effect and the hemolysis matrix effect are not interfered, the accuracy and precision are high, the dosage is small, and the cost is low. The method can be used for clinical large-batch sample analysis. 本发明属于医药检测技术领域，具体公开了一种液质联用测定人血浆中氟氢可的松浓度的方法，采取液液萃取的方法提取血