Method for detecting contents of rutin, isoquercitrin and rosmarinic acid in anchusa asiatica or anchusa asiatica extract

The invention provides a method for detecting the contents of rutin, isoquercitrin and rosmarinic acid in anchusa officinalis or anchusa officinalis extract, and the method comprises the following steps: carrying out liquid chromatography detection on the anchusa officinalis extract or the extract obtained by extracting the anchusa officinalis, and determining the contents of rutin, isoquercitrin and rosmarinic acid based on the detection result, wherein a mobile phase adopted in the liquid chromatography detection is acetonitrile and a phosphoric acid solution. The method provided by the invention can be used for accurately determining the contents of rutin, isoquercitrin and rosmarinic acid in the origanum vulgare or the extract thereof, and has the advantages of strong specificity, high accuracy, good reproducibility, high sensitivity, good durability, obvious improvement of chromatographic peak shape and trailing phenomenon, and the like. The method can be used for quality control of the anchusa or the an