SSR core primer group for purity identification of cauliflower hybrid as well as screening method and application of SSR core primer group
The invention discloses an SSR (Simple Sequence Repeat) core primer group for purity identification of cauliflower hybrids as well as a screening method and application of the SSR core primer group, and belongs to the technical field of molecular markers. The SSR core primer group comprises BoGMS062...
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Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses an SSR (Simple Sequence Repeat) core primer group for purity identification of cauliflower hybrids as well as a screening method and application of the SSR core primer group, and belongs to the technical field of molecular markers. The SSR core primer group comprises BoGMS0624, OI11G11 and BoGMS0941, and the SSR core primer group comprises BoGMS0624, OI11G11 and And the alternative core primer group comprises the following components: BoE607, BoE761, BoGMS2431, BoGMS0808 and BoGMS1530. According to the method, DNA fingerprint analysis is performed on 70 hybrids of cauliflower and broccoli, so that evaluation indexes of core primers suitable for purity identification of the hybrids are determined, a set of primers for purity identification of the cauliflower hybrids are determined, the purity of the cauliflower hybrids is identified by using the fewest primers with wide genome coverage, the workload is greatly reduced, and the method is suitable for industrial production. The detection |
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