Molecular marker for identifying hereditary sex of haliotis diversicolor and application of molecular marker

The invention discloses a molecular marker for identifying hereditary sex of haliotis diversicolor and application of the molecular marker. The molecular marker shows insertion/deletion length polymorphism of a nucleotide sequence as shown in SEQ ID NO: 1. One of a pair of homologous chromosomes of...

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Hauptverfasser: CAI MINGYI, XU YANGRU, WENG XIAOXIA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a molecular marker for identifying hereditary sex of haliotis diversicolor and application of the molecular marker. The molecular marker shows insertion/deletion length polymorphism of a nucleotide sequence as shown in SEQ ID NO: 1. One of a pair of homologous chromosomes of a haliotis diversicolor individual is lack of the SEQ ID NO: 1 nucleotide sequence, and the genetic sex is male; a pair of homologous chromosomes of a haliotis diversicolor individual do not lack the SEQ ID NO: 1 nucleotide sequence, and the genetic sex is female. During detection, PCR amplification is performed by adopting SEQ ID NO: 2 and SEQ ID NO: 3 as primers, and when an amplification product is two fragments, the lengths of the two fragments are 266bp and 243bp respectively, the genetic sex of the abalone to be detected is male; when the amplification product only has one fragment and the length is 266bp, the genetic sex of the abalone to be detected is female. The method is suitable for both before and afte