Kit for detecting human HER-2 gene amplification as well as application and working method thereof

The invention relates to a kit for detecting human HER-2 gene amplification as well as application and a working method thereof, the kit comprises a primer pair and a fluorescent probe for detecting the HER-2 gene, a primer pair and a fluorescent probe for detecting a reference gene EIF2C1, a 2 * ddPCR premixed solution, a negative quality control product and a positive quality control product, and the primer pair and the fluorescent probe for detecting the HER-2 gene, a nucleotide sequence of a forward primer of the kit is 5 '-TACGTGCTCATGCTCACA-3', a nucleotide sequence of a reverse primer of the kit is 5 '-GGTGCCTGCACAATCC-3', and a nucleotide sequence of a fluorescent probe of the kit is 5 '-AGTGAGGCAGGTCCCACTGC-3'. The kit disclosed by the invention can be used for detecting the copy number of the human HER-2 gene on the basis of a digital PCR (Polymerase Chain Reaction) technology, has the advantages of absolute quantification, simplicity and convenience in operation, high accuracy, good specificity, lo