Method for synthesizing L-fructose through whole-cell transformation

The invention relates to the technical field of biology, in particular to a method for synthesizing L-fructose by utilizing whole-cell transformation, a reaction system in the method comprises substrate glycerol, L-glyceraldehyde, pyrophosphoric acid and wet thalli of induced recombinant escherichia...

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Bibliographische Detailangaben
Hauptverfasser:	LI ZIJIE, CHEN ZHOU, ZHU LIPING, CHEN KECAI, GAO XIAODONG, HU YANGFAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of biology, in particular to a method for synthesizing L-fructose by utilizing whole-cell transformation, a reaction system in the method comprises substrate glycerol, L-glyceraldehyde, pyrophosphoric acid and wet thalli of induced recombinant escherichia coli, and the L-fructose can be obtained after the reaction system is subjected to catalytic reaction. By utilizing the method, the yield of the L-fructose can be improved. 本发明涉及生物技术领域，尤其涉及一种利用全细胞转化合成L-果糖的方法，方法中反应体系包括底物甘油、L-甘油醛、焦磷酸，经过诱导后的重组大肠杆菌的湿菌体，所述反应体系经过催化反应后即可得到L-果糖。利用该方法可提高L-果糖的产率。