GRNA (guide Ribonucleic Acid) sequence for rapidly improving resistance of buds on spike and application thereof

The invention belongs to the technical field of genetic breeding, and particularly relates to a gRNA sequence for rapidly improving the resistance of buds on ears and application of the gRNA sequence. Specifically, the invention provides 15 gRNA sequences as shown in SEQ ID NO.1 to SEQ ID NO.15, and the 15 gRNA sequences are applied to perform genome editing on a target sequence, so that crop seeds with longer dormancy period are prepared. Theoretically, the method can quickly realize the improvement of the on-ear bud resistance, prolongs the germination period of wheat seeds, and provides an efficient breeding mode for improving the on-ear bud resistance. According to the method disclosed by the invention, the high-ear bud resistance character can be superposed in a good variety only through one-time genetic transformation, the breeding period is short, the technology is simple and easy to implement, and the cost is lower; the cas9 exogenous DNA sequence of the obtained final product can be separated through