Tissue culture method of North China scabiosa

The invention discloses a tissue culture method of northern China potted flowers, which comprises the following steps: step 1, disinfection: firstly using 75% alcohol and then using 2% sodium hypochlorite for disinfection; step 2, callus induction: cutting the disinfected leaves into squares, and inoculating the squares into a callus induction culture medium to induce callus, and step 3, callus differentiation induction: cutting the callus into cube small blocks, inoculating the cube small blocks into a callus induction differentiation culture medium to differentiate adventitious buds, inoculating the adventitious buds of 6-8cm into a root culture medium, and culturing the adventitious buds in the root culture medium. Adventitious buds are promoted to root to form regenerated seedlings. 本发明公开了一种华北蓝盆花的组织培养方法，包括以下步骤：步骤1、消毒，先用75％酒精，再用2％次氯酸钠消毒；步骤2、诱导愈伤组织：将消毒后的叶片剪成正方形接种到诱导愈伤组织培养基中诱导出愈伤组织，步骤3、诱导愈伤组织分化，将愈伤组织切成正方体小块，接种到诱导愈伤组织分化培养基中，分化出不定芽，将6-8cm的不定芽接种于根培养基中，促进不定芽生根形成再生苗。