HPLC (High Performance Liquid Chromatography) detection method for content of related substances in p-bromoanisole

The invention discloses an HPLC (High Performance Liquid Chromatography) detection method for the content of related substances in p-bromoanisole, which comprises the following steps: respectively injecting a sample solution and a reference substance solution, and detecting the content of p-bromophenol and o-bromoanisole in p-bromoanisole by an HPLC method, and the HPLC chromatographic conditions are as follows: a chromatographic column is an octadecyl bonded silica gel chromatographic column; a mobile phase: taking a 0.01 mol/L sodium dihydrogen phosphate solution as a mobile phase A and acetonitrile as a mobile phase B; carrying out elution; the column temperature is 25-40 DEG C; the flow rate is 1.0 ml/min; the sample injection volume is 1-20 microliters; and the detector is a UV (ultraviolet) detector with the wavelength of 210-230 nm. The HPLC detection method provided by the invention has good system applicability, detection limit, quantitation limit, durability, precision, accuracy and recovery rate, i