Method for producing L-ornithine by immobilized enzyme method
The invention discloses a method for producing L-ornithine by an immobilized enzyme method, which comprises the following steps: (1) inoculating a seed solution of a human recombinant arginase I producing strain into a fermentation culture medium for fermentation culture, cooling, adding IPTG (isopr...
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| creator | CAO HUAJIE GUO YONGSHENG XIE PEI YUE MINGRUI |
| description | The invention discloses a method for producing L-ornithine by an immobilized enzyme method, which comprises the following steps: (1) inoculating a seed solution of a human recombinant arginase I producing strain into a fermentation culture medium for fermentation culture, cooling, adding IPTG (isopropyl-beta-d-thiogalactoside) into the system, and performing induced culture for 20-28 hours; (2) carrying out bacterium breaking treatment on the culture solution subjected to induced culture, passing through a ceramic membrane, and collecting filtrate; hanging the filtrate on a column; and (3) enabling a substrate solution containing L-arginine to flow through the nickel column with the hung column for biotransformation, and collecting an effluent transformation solution, so as to produce L-ornithine. According to the invention, a novel human recombinant arginase I producing strain is firstly constructed, and a fermentation process is optimized, so that the yield and enzyme activity of the human recombinant argin |
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According to the invention, a novel human recombinant arginase I producing strain is firstly constructed, and a fermentation process is optimized, so that the yield and enzyme activity of the human recombinant argin</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220318&DB=EPODOC&CC=CN&NR=114196712A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220318&DB=EPODOC&CC=CN&NR=114196712A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CAO HUAJIE</creatorcontrib><creatorcontrib>GUO YONGSHENG</creatorcontrib><creatorcontrib>XIE PEI</creatorcontrib><creatorcontrib>YUE MINGRUI</creatorcontrib><title>Method for producing L-ornithine by immobilized enzyme method</title><description>The invention discloses a method for producing L-ornithine by an immobilized enzyme method, which comprises the following steps: (1) inoculating a seed solution of a human recombinant arginase I producing strain into a fermentation culture medium for fermentation culture, cooling, adding IPTG (isopropyl-beta-d-thiogalactoside) into the system, and performing induced culture for 20-28 hours; (2) carrying out bacterium breaking treatment on the culture solution subjected to induced culture, passing through a ceramic membrane, and collecting filtrate; hanging the filtrate on a column; and (3) enabling a substrate solution containing L-arginine to flow through the nickel column with the hung column for biotransformation, and collecting an effluent transformation solution, so as to produce L-ornithine. 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According to the invention, a novel human recombinant arginase I producing strain is firstly constructed, and a fermentation process is optimized, so that the yield and enzyme activity of the human recombinant argin</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for producing L-ornithine by immobilized enzyme method |
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