Method for producing L-ornithine by immobilized enzyme method

The invention discloses a method for producing L-ornithine by an immobilized enzyme method, which comprises the following steps: (1) inoculating a seed solution of a human recombinant arginase I producing strain into a fermentation culture medium for fermentation culture, cooling, adding IPTG (isopropyl-beta-d-thiogalactoside) into the system, and performing induced culture for 20-28 hours; (2) carrying out bacterium breaking treatment on the culture solution subjected to induced culture, passing through a ceramic membrane, and collecting filtrate; hanging the filtrate on a column; and (3) enabling a substrate solution containing L-arginine to flow through the nickel column with the hung column for biotransformation, and collecting an effluent transformation solution, so as to produce L-ornithine. According to the invention, a novel human recombinant arginase I producing strain is firstly constructed, and a fermentation process is optimized, so that the yield and enzyme activity of the human recombinant argin