Antihypertensive peptide as well as preparation method and application thereof

The invention belongs to the technical field of biology, and particularly relates to antihypertensive peptide and application thereof in medicine, functional food and health food. Spirulina is used as a raw material, protease K is used for conducting enzymolysis on spirulina protein, and polypeptide obtained after enzymolysis is subjected to gel chromatography and reversed-phase chromatography separation to obtain the oligopeptide with the sequence being TVLYEH. Researches show that the polypeptide has significant angiotensin converting enzyme (ACE) inhibitory activity, IC50 is 2.59 [mu] M, and no obvious cytotoxicity exists. The invention provides the method for preparing the antihypertensive peptide, and the high-activity oligopeptide is successfully identified and has good development and utilization prospects. 本发明属于生物技术领域，具体涉及一种降压肽及其在药物、功能性食品保健食品中的应用。本发明以螺旋藻为原料，使用蛋白酶K对螺旋藻蛋白进行酶解，酶解后的多肽经凝胶层析和反相色谱分离获得序列为TVLYEH的低聚肽。研究表明，该多肽具有显著的血管紧张素转化酶(ACE)抑制活性，IC50为2.59μM，且没有明显的细胞毒性。本发明提供了一种制备降压肽的方法，并成功鉴定到一个高活性的低聚肽，具有良好的开发利