Diluting method of digital micro-fluidic liquid drops

The invention provides a digital microfluidic droplet dilution method, which is located in a driving electrode unit array constructed by a digital microfluidic platform, and comprises the following steps: S1, respectively separating sample droplets and solvent droplets with specific sizes from a sam...

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Bibliographische Detailangaben
Hauptverfasser: CHEN RIFEI, ZHANG CHENGZHI, LI YUYAN, XING YARU, LIU YU, CHENG XIN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention provides a digital microfluidic droplet dilution method, which is located in a driving electrode unit array constructed by a digital microfluidic platform, and comprises the following steps: S1, respectively separating sample droplets and solvent droplets with specific sizes from a sample pool and a blank solvent pool, the sample liquid drops and the solvent liquid drops are first liquid drops; s2, moving the sample liquid drops and the solvent liquid drops to the same electrode position, and mixing to form mixed liquid drops; s3, uniformly mixing the mixed liquid drops; and S4, splitting the mixed liquid drop into two liquid drops, wherein the two liquid drops are both second liquid drops. According to the invention, the volume of liquid drops can be controlled to be small, and rare samples or samples in small demand can be diluted; the liquid drop moving speed is high, so that the consumed time can be greatly reduced; meanwhile, a large number of liquid drops are controlled, and high-flux dilu