Esterase sourced from Antarctic soil as well as coding gene and application of esterase

The invention provides esterase derived from Antarctic soil as well as a coding gene and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. According to the invention, an esterase-producing strain Pseudomonas sp.Soil2 is separated from soil in Jiangwa...

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Bibliographische Detailangaben
Hauptverfasser: LIU XIAOYU, SUN SHIZE, CHU TIANGE, CAO WEITONG, ZHOU MINGYANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides esterase derived from Antarctic soil as well as a coding gene and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. According to the invention, an esterase-producing strain Pseudomonas sp.Soil2 is separated from soil in Jiangwang Island of Antarctic, a gene segment Est-soil2 for coding esterase in a genome of the strain and a nucleotide sequence of the Est-soil2 are determined, and a gene for coding the esterase Est-soil2 derived from Antarctic soil is successfully cloned, so that the esterase-producing strain Pseudomonas sp.Soil2 can be used for preparing the esterase-producing strain Pseudomonas sp.Soil2, and the esterase-producing strain Pseudomonas sp.Soil2. An expression vector containing the esterase gene Est-soil2 derived from the Antarctic soil and an escherichia coli recombinant cell containing the expression vector are constructed. The esterase belongs to cold adaptive esterase, has good catalytic performance within the range o