Recombinant pichia pastoris for heterologous expression of myrosinase and application of recombinant pichia pastoris in preparation of sulforaphane

The invention discloses recombinant pichia pastoris for heterologous expression of myrosinase and application of the recombinant pichia pastoris in preparation of sulforaphane, and belongs to the technical field of bioengineering. The method comprises the following steps: firstly, constructing a recombinant strain capable of heterologous expression of myrosinase, fermenting the recombinant strain to produce myrosinase, and catalyzing glucoraphanin through immobilized enzyme to generate sulforaphane. When the BMYR containing a cabbage aphid source and prepared from the recombinant Pichia pastoris P.pastoris X33/pPICZ alpha-BMYR provided by the invention is used for preparing the sulforaphane in a 3L fermentation tank, the yield reaches 0.91 g/L, the molar conversion rate of glucoraphane reaches 90%, and the method for preparing the sulforaphane by the biological method provided by the invention has the advantages of mild reaction conditions, simple process, easiness in control and the like, and is suitable for