Primer and kit for detecting coronavirus
The invention provides a universal specific PCR (Polymerase Chain Reaction) detection primer pair for detecting coronavirus, and a method for carrying out specific PCR detection on the coronavirus SADS-CoV virus and SARS-CoV-2 virus by utilizing the primer pair. According to the primer pair for perf...
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| creator | LIU XIAOFEI ZHANG ZHOU YUAN XIANGFEN WU SHAOQIANG WANG CAIXIA LIN XIANGMEI LYU JIZHOU |
| description | The invention provides a universal specific PCR (Polymerase Chain Reaction) detection primer pair for detecting coronavirus, and a method for carrying out specific PCR detection on the coronavirus SADS-CoV virus and SARS-CoV-2 virus by utilizing the primer pair. According to the primer pair for performing specific detection on the coronavirus, provided by the invention, when the microdroplet digital RT-PCR is utilized for amplification, the SADS-CoV virus and the SARS-CoV-2 virus can be specifically detected at the same time, and the SADS-CoV or SARS-CoV-2 nucleic acid can be rapidly and accurately subjected to absolute quantification while the detection cost is greatly reduced.
本发明提供了一种用于检测冠状病毒的通用特异性PCR检测引物对，以及利用该引物对冠状病毒SADS-CoV病毒和SARS-CoV-2病毒进行特异性PCR检测方法。本申请提供的用于对冠状病毒进行特异性检测的引物对，在利用微滴数字RT-PCR进行扩增时，能够特异性地同时检测出SADS-CoV病毒和SARS-CoV-2病毒，在大幅降低检测成本的同时，可以快速、精准的对SADS-CoV或SARS-CoV-2核酸进行绝对定量。 |
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本发明提供了一种用于检测冠状病毒的通用特异性PCR检测引物对，以及利用该引物对冠状病毒SADS-CoV病毒和SARS-CoV-2病毒进行特异性PCR检测方法。本申请提供的用于对冠状病毒进行特异性检测的引物对，在利用微滴数字RT-PCR进行扩增时，能够特异性地同时检测出SADS-CoV病毒和SARS-CoV-2病毒，在大幅降低检测成本的同时，可以快速、精准的对SADS-CoV或SARS-CoV-2核酸进行绝对定量。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2022</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220304&DB=EPODOC&CC=CN&NR=114134252A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20220304&DB=EPODOC&CC=CN&NR=114134252A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU XIAOFEI</creatorcontrib><creatorcontrib>ZHANG ZHOU</creatorcontrib><creatorcontrib>YUAN XIANGFEN</creatorcontrib><creatorcontrib>WU SHAOQIANG</creatorcontrib><creatorcontrib>WANG CAIXIA</creatorcontrib><creatorcontrib>LIN XIANGMEI</creatorcontrib><creatorcontrib>LYU JIZHOU</creatorcontrib><title>Primer and kit for detecting coronavirus</title><description>The invention provides a universal specific PCR (Polymerase Chain Reaction) detection primer pair for detecting coronavirus, and a method for carrying out specific PCR detection on the coronavirus SADS-CoV virus and SARS-CoV-2 virus by utilizing the primer pair. According to the primer pair for performing specific detection on the coronavirus, provided by the invention, when the microdroplet digital RT-PCR is utilized for amplification, the SADS-CoV virus and the SARS-CoV-2 virus can be specifically detected at the same time, and the SADS-CoV or SARS-CoV-2 nucleic acid can be rapidly and accurately subjected to absolute quantification while the detection cost is greatly reduced.
本发明提供了一种用于检测冠状病毒的通用特异性PCR检测引物对，以及利用该引物对冠状病毒SADS-CoV病毒和SARS-CoV-2病毒进行特异性PCR检测方法。本申请提供的用于对冠状病毒进行特异性检测的引物对，在利用微滴数字RT-PCR进行扩增时，能够特异性地同时检测出SADS-CoV病毒和SARS-CoV-2病毒，在大幅降低检测成本的同时，可以快速、精准的对SADS-CoV或SARS-CoV-2核酸进行绝对定量。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2022</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNAIKMrMTS1SSMxLUcjOLFFIyy9SSEktSU0uycxLV0jOL8rPSyzLLCot5mFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8c5-hoYmhsYmRqZGjsbEqAEAyE4oQA</recordid><startdate>20220304</startdate><enddate>20220304</enddate><creator>LIU XIAOFEI</creator><creator>ZHANG ZHOU</creator><creator>YUAN XIANGFEN</creator><creator>WU SHAOQIANG</creator><creator>WANG CAIXIA</creator><creator>LIN XIANGMEI</creator><creator>LYU JIZHOU</creator><scope>EVB</scope></search><sort><creationdate>20220304</creationdate><title>Primer and kit for detecting coronavirus</title><author>LIU XIAOFEI ; ZHANG ZHOU ; YUAN XIANGFEN ; WU SHAOQIANG ; WANG CAIXIA ; LIN XIANGMEI ; LYU JIZHOU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN114134252A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2022</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU XIAOFEI</creatorcontrib><creatorcontrib>ZHANG ZHOU</creatorcontrib><creatorcontrib>YUAN XIANGFEN</creatorcontrib><creatorcontrib>WU SHAOQIANG</creatorcontrib><creatorcontrib>WANG CAIXIA</creatorcontrib><creatorcontrib>LIN XIANGMEI</creatorcontrib><creatorcontrib>LYU JIZHOU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU XIAOFEI</au><au>ZHANG ZHOU</au><au>YUAN XIANGFEN</au><au>WU SHAOQIANG</au><au>WANG CAIXIA</au><au>LIN XIANGMEI</au><au>LYU JIZHOU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Primer and kit for detecting coronavirus</title><date>2022-03-04</date><risdate>2022</risdate><abstract>The invention provides a universal specific PCR (Polymerase Chain Reaction) detection primer pair for detecting coronavirus, and a method for carrying out specific PCR detection on the coronavirus SADS-CoV virus and SARS-CoV-2 virus by utilizing the primer pair. According to the primer pair for performing specific detection on the coronavirus, provided by the invention, when the microdroplet digital RT-PCR is utilized for amplification, the SADS-CoV virus and the SARS-CoV-2 virus can be specifically detected at the same time, and the SADS-CoV or SARS-CoV-2 nucleic acid can be rapidly and accurately subjected to absolute quantification while the detection cost is greatly reduced.
本发明提供了一种用于检测冠状病毒的通用特异性PCR检测引物对，以及利用该引物对冠状病毒SADS-CoV病毒和SARS-CoV-2病毒进行特异性PCR检测方法。本申请提供的用于对冠状病毒进行特异性检测的引物对，在利用微滴数字RT-PCR进行扩增时，能够特异性地同时检测出SADS-CoV病毒和SARS-CoV-2病毒，在大幅降低检测成本的同时，可以快速、精准的对SADS-CoV或SARS-CoV-2核酸进行绝对定量。</abstract><oa>free_for_read</oa></addata></record> |
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| language | chi ; eng |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Primer and kit for detecting coronavirus |
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