Primer and kit for detecting coronavirus

The invention provides a universal specific PCR (Polymerase Chain Reaction) detection primer pair for detecting coronavirus, and a method for carrying out specific PCR detection on the coronavirus SADS-CoV virus and SARS-CoV-2 virus by utilizing the primer pair. According to the primer pair for performing specific detection on the coronavirus, provided by the invention, when the microdroplet digital RT-PCR is utilized for amplification, the SADS-CoV virus and the SARS-CoV-2 virus can be specifically detected at the same time, and the SADS-CoV or SARS-CoV-2 nucleic acid can be rapidly and accurately subjected to absolute quantification while the detection cost is greatly reduced. 本发明提供了一种用于检测冠状病毒的通用特异性PCR检测引物对，以及利用该引物对冠状病毒SADS-CoV病毒和SARS-CoV-2病毒进行特异性PCR检测方法。本申请提供的用于对冠状病毒进行特异性检测的引物对，在利用微滴数字RT-PCR进行扩增时，能够特异性地同时检测出SADS-CoV病毒和SARS-CoV-2病毒，在大幅降低检测成本的同时，可以快速、精准的对SADS-CoV或SARS-CoV-2核酸进行绝对定量。