Packaging and detection method of SARS-CoV-2 pseudovirus system capable of simultaneously expressing RFP and luciferase

The invention belongs to the technical field of biology, and relates to a method for packaging and detecting an SARS-CoV-2 pseudovirus system capable of simultaneously expressing RFP (Restriction Fragment Protein) and luciferase. The SARS-CoV-2 pseudovirus obtained by packaging has no autonomous replication ability and good safety, not only can express the SARS-CoV-2 Spike protein on the surface, but also carries RFP and a luciferase luciferase reporter gene at the same time, can be subjected to qualitative and quantitative analysis by using a flow method and a microplate reader spectrophotometric analysis method at the same time, and has good application prospects. The kit can be used for SARS-CoV-2 neutralizing antibody detection, SARS-CoV-2 receptor and drug screening, vaccine effect evaluation and the like. 本发明属于生物技术领域，涉及一种同时表达RFP和luciferase的SARS-CoV-2假病毒系统的包装和检测方法。本发明包装得到的SARS-CoV-2假病毒无自主复制能力，安全性好，不仅表面能表达SARS-CoV-2 Spike蛋白，而且该病毒同时携带RFP和luciferase荧光素酶报告基因，可以同时利用流式方法和酶标仪分光光度分析方法进行定性和定量分析，可用于SARS-CoV-2中和抗体检