GRNA (guide Ribonucleic Acid) sequence for rapidly increasing anthocyanin content of crops and application thereof

The invention belongs to the technical field of genetic breeding, and particularly relates to a gRNA sequence for rapidly increasing the anthocyanin content of crops and application of the gRNA sequence. Specifically, 20 gRNA sequences as shown in SEQ ID NO.1 to SEQ ID NO.20 are provided and are used for genome editing of targeted 200bp, rice color improvement of white wheat can be rapidly achieved, accumulation of nutrient anthocyanin is further promoted, red wheat seed resources are created, and high-yield and high-quality red wheat varieties are cultivated. According to the scheme, complicated hybridization polymerization and backcross are not needed, only one-time genetic transformation is needed, the high anthocyanin character of the red wheat can be overlaid in the excellent white wheat variety, the technology is simple and easy to implement, the cost is lower, and the breeding period is short. 本发明属于遗传育种技术领域，具体涉及快速提高农作物花青素含量的gRNA序列及其应用。具体的，本发明提供SEQ ID NO.1至SEQ ID NO.20所示的20个gRNA序列，并将其用于对靶向200bp进行基因组编辑，可