Inactivation method of mycoplasma hyopneumoniae

The invention discloses a method for inactivating mycoplasma hyopneumoniae. The method comprises the following steps: adding diethyleneimine (BEI) into a mycoplasma hyopneumoniae bacterial liquid subjected to ultrasonication, and inactivating for 24-48 hours. The inactivation method of the mycoplasm...

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Bibliographische Detailangaben
Hauptverfasser:	ZHENG QINQIN, LIN YAN, CHEN LIQUN, YUE FENGXIONG, XIANG CONGYING, LIU HANPING, XIA JIAXIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method for inactivating mycoplasma hyopneumoniae. The method comprises the following steps: adding diethyleneimine (BEI) into a mycoplasma hyopneumoniae bacterial liquid subjected to ultrasonication, and inactivating for 24-48 hours. The inactivation method of the mycoplasma hyopneumoniae is thorough in antigen inactivation and short in time consumption, and a vaccine prepared from the inactivated mycoplasma hyopneumoniae is high in protein purity, less in immune stress, high in immune response and suitable for market popularization and application. 本发明公开了一种猪肺炎支原体的灭活方法，它包括向经超声破碎的猪肺炎支原体菌液中添加二乙烯亚胺(BEI)灭活24～48h。本发明猪肺炎支原体的灭活方法，抗原灭活彻底且耗时短，用本发明灭活的猪肺炎支原体制备的疫苗，蛋白纯度高、免疫应激少、免疫应答高，适宜市场推广使用。