Method for identifying oleanolic acid of Xiaokang granules

The invention discloses a Xiaokang granule oleanolic acid identification method which comprises the following steps: (1) preparing a test solution, namely grinding a Xiaokang granule sample, adding trichloromethane, heating, refluxing and extracting, evaporating an extracting solution to dryness, ad...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: GE YUE, ZHANG XIABING, JIANG TINGFENG, YANG YANLI, BAI BAORUI, LIU GUOLIN
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention discloses a Xiaokang granule oleanolic acid identification method which comprises the following steps: (1) preparing a test solution, namely grinding a Xiaokang granule sample, adding trichloromethane, heating, refluxing and extracting, evaporating an extracting solution to dryness, adding a mixed solution of absolute ethyl alcohol and trichloromethane into residues, heating and dissolving to obtain the test solution; (2) preparing a reference substance solution: taking an oleanolic acid reference substance, and adding ethanol to prepare a solution as the reference substance solution; (3) identification: adopting thin layer chromatography, sucking the reference substance solution and the test sample solution, respectively dripping the reference substance solution and the test sample solution on the same silica gel G thin layer plate, developing, taking out, airing, spraying a sulfuric acid ethanol solution by taking a mixed solution of cyclohexane and ethyl acetate as a developing solvent, and h