Method for identifying oleanolic acid of Xiaokang granules
The invention discloses a Xiaokang granule oleanolic acid identification method which comprises the following steps: (1) preparing a test solution, namely grinding a Xiaokang granule sample, adding trichloromethane, heating, refluxing and extracting, evaporating an extracting solution to dryness, ad...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a Xiaokang granule oleanolic acid identification method which comprises the following steps: (1) preparing a test solution, namely grinding a Xiaokang granule sample, adding trichloromethane, heating, refluxing and extracting, evaporating an extracting solution to dryness, adding a mixed solution of absolute ethyl alcohol and trichloromethane into residues, heating and dissolving to obtain the test solution; (2) preparing a reference substance solution: taking an oleanolic acid reference substance, and adding ethanol to prepare a solution as the reference substance solution; (3) identification: adopting thin layer chromatography, sucking the reference substance solution and the test sample solution, respectively dripping the reference substance solution and the test sample solution on the same silica gel G thin layer plate, developing, taking out, airing, spraying a sulfuric acid ethanol solution by taking a mixed solution of cyclohexane and ethyl acetate as a developing solvent, and h |
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