Method for detecting phospholipid in hemoglobin oxygen carrier

The invention relates to the technical field of phospholipid detection, in particular to a method for detecting phospholipid in a hemoglobin oxygen carrier. According to the method, phospholipids in a sample to be tested are extracted and then subjected to alkaline saponification, reaction products including ethanolamine and serine are subjected to derivatization reaction with p-toluenesulfonyl chloride (PTSC), and derivative products are detected at 240 nm after being separated through reversed-phase high-performance liquid chromatography. According to the method, a large number of organic solvents which are unfriendly to the environment when phospholipids are determined through normal-phase chromatography are abandoned, and the problems that the normal-phase chromatography is lack of chromophores, and the detection sensitivity is high are solved. The method can be used for conventional items of phospholipids in hemoglobin oxygen carriers. 本发明涉及磷脂检测技术领域，具体涉及一种血红蛋白氧载体中磷脂的检测方法。供试样品中的磷脂经提取后碱性皂化，反应产物乙醇胺和丝氨酸与对甲苯磺