Purification preparation method of sulfopeptide indicator

The invention discloses a purification preparation method of a sulfopeptide indicator, and relates to purification preparation of reagents. A rapid liquid phase preparative chromatography system is adopted to detect and separate the sulfopeptide indicator and impurities in the sulfopeptide indicator, and a rotary evaporator and a nitrogen blowing concentrator are adopted to recover and prepare the purified sulfopeptide indicator. The method comprises the following steps: weighing a sulfopeptide indicator, dissolving the sulfopeptide indicator in N, N-dimethylformamide, carrying out ultrasonic treatment, filtering, and determining and separating in preparative liquid chromatography; and collecting the separated purified sample, transferring the purified sample to a rotary evaporator by using acetonitrile, carrying out rotary evaporation to a certain volume, transferring the sample to a nitrogen blowing instrument by using acetonitrile, carrying out nitrogen blowing to a certain volume, transferring the sample