Insect-resistant fusion gene M2CryAb-VIP3A as well as expression vector, product and application thereof

The invention relates to an insect-resistant fusion gene M2CryAb-VIP3A as well as an expression vector, a product and application of the insect-resistant fusion gene M2CryAb-VIP3A, and aims to solve the technical problem that the existing Bt fusion gene is difficult to give consideration to both bio...

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Bibliographische Detailangaben
Hauptverfasser:	LI WENLAN, YUE RUNQING, LI WENCAI, MENG ZHAODONG, LU SHOUPING, YU YANLI, ZENG TINGRU, ZHAO MENG, ZHANG QINGWEI, SUN QI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to an insect-resistant fusion gene M2CryAb-VIP3A as well as an expression vector, a product and application of the insect-resistant fusion gene M2CryAb-VIP3A, and aims to solve the technical problem that the existing Bt fusion gene is difficult to give consideration to both biological safety and efficient insect killing. The nucleotide sequence of the insect-resistant fusion gene M2CryAb-VIP3A is as shown in SEQ ID NO. 4, and the corresponding protein sequence of the insect-resistant fusion gene M2CryAb-VIP3A is as shown in SEQ ID NO. 5. The fusion gene does not contain a sensitizer sequence and can be efficiently expressed, the biological safety and the insecticidal effect are both considered, and an expression product of the fusion gene has excellent resistance to pests such as ostrinia nubilalis and spodoptera frugiperda and is wider in insecticidal spectrum. After the M2CryAb-VIP3A gene is introduced into corn, a new crop variety which is resistant to stem borers and also resistant t