Nucleic acid methylation cytosine conversion method

The invention provides a nucleic acid methylation cytosine conversion method. The method is characterized by comprising the following steps: (1) carrying out TET enzyme oxidation treatment on a DNA or RNA sample; (2) adding pyridine borane into a reaction system for treatment; and (3) recovering DNA...

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Bibliographische Detailangaben
Hauptverfasser:	LIU QIAN, SONG DONGLIANG, CAO ZHEN, HOU CE, WEI LEI, CHEN JINGJING, WANG MAN, SUN RUI, JIANG AO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a nucleic acid methylation cytosine conversion method. The method is characterized by comprising the following steps: (1) carrying out TET enzyme oxidation treatment on a DNA or RNA sample; (2) adding pyridine borane into a reaction system for treatment; and (3) recovering DNA or RNA in the reaction system by magnetic beads. According to the present invention, the whole process of the nucleic acid methylation cytosine conversion method only comprises three steps such as TET enzyme oxidation, pyridine borane treatment and DNA magnetic bead recovery, such that the conversion efficiency of the TAPS is improved, the operation difficulty and the operation flow of the TAPS are simplified, and the nucleic acid methylation cytosine conversion method is named as Enhanced TET-assisted pyridine borane sequencing (eTAPS), the eTAPS has the advantages of high conversion rate, small DNA damage, simplicity in operation, convenience in automation and the like, and in addition, we find that the eTAPS al