Primer probe design method for miRNA detection, detection composition and kit

The invention belongs to the field of molecular biological detection, particularly belongs to the field of miRNA detection, and more particularly relates to one-step detection of miRNA. The invention provides a primer and probe design method for miRNA detection, a detection composition comprising a primer and a probe designed by the primer and probe design method, and a detection kit comprising the detection composition. By using the primer and the probe designed by the method disclosed by the invention, miRNA detection can be carried out by adopting the one-step method, non-specific amplification is avoided, the sensitivity is high and reaches 1000 copies/mL, and the specificity is good. The operation is simpler and safer, and the result is more credible. 本发明属于分子生物学检测领域，具体地，属于miRNA检测领域，更具体地，涉及miRNA的一步法检测。本发明提供了一种miRNA检测的引物和探针设计方法，包括该引物和探针设计方法设计的引物和探针的检测组合物，包括该检测组合物的检测试剂盒。使用本发明方法设计的引物和探针，能够使得miRNA的检测可以采用一步法进行，不会出现非特异性扩增，并且其灵敏度高，达到1000拷贝/mL，特异性好。操作更加简单、安全，结果更为可信。