Recyclable hollow cantilever probe front end loading method and cleaning method

The invention discloses a recyclable hollow cantilever probe front end loading method and cleaning method, and solves the problems of low liquid utilization rate and tedious cleaning steps of a hollow cantilever probe in an application scene of high operating liquid cost and low using amount. The front end loading method comprises the following steps: filtering and degassing liquid to be operated; transferring the liquid drops onto a transparent substrate; contacting the calibrated probe with the liquid drop; applying negative pressure pulse through the pressure controller to complete front-end loading of the liquid. The invention also provides the cleaning method after front-end loading. The cleaning method comprises the following steps: immersing the probe subjected to the test in a cleaning solution for a moment, washing with ultrapure water to complete external cleaning, then immersing the probe in a sodium hypochlorite solution and an ethanol water solution in sequence, alternately applying positive pres