Construction of RIPLET knockout cell line and application of RIPLET knockout cell line as picornaviridae virus vaccine production cell line

The invention belongs to the field of gene engineering, and particularly relates to construction of an RIPLET knockout cell line and application of the RIPLET knockout cell line as a picornaviridae virus vaccine production cell line. Firstly, it is found that inhibition of RIPLET gene expression in...

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Bibliographische Detailangaben
Hauptverfasser:	QI XIAOLAN, ZHANG XIANGLE, CAO WEIJUN, LIU XIANGTAO, ZHU ZIXIANG, YANG FAN, CHEN WENZHE, ZHANG KESHAN, TANG WENDA, GU FENGXING, ZHENG HAIXUE, LI PAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the field of gene engineering, and particularly relates to construction of an RIPLET knockout cell line and application of the RIPLET knockout cell line as a picornaviridae virus vaccine production cell line. Firstly, it is found that inhibition of RIPLET gene expression in host cells can promote replication of picornaviridae viruses, especially foot and mouth disease viruses and Seneca viruses. Secondly, the invention provides sgRNA specifically targeting RIPLET. The sgRNA can specifically target the RIPLET gene, the knockout of the RIPLET gene is realized by combining a CRISPR-Cas9 technology, and an obtained monoclonal cell line can significantly promote the replication of picornaviridae viruses, especially foot-and-mouth disease viruses and Seneca viruses. The production quantity and the antigen expression quantity of picornaviridae virus vaccines are improved, and the cell line can be used as a picornaviridae virus vaccine production cell line and has a wide application prospect.