METHOD FOR SEQUENCING POLYNUCLEOTIDES

The present invention relates to improvements in high throughput nucleic acid sequencing methods, and in particular to improvements in methods for performing extension reactions during paired sequencing. The present invention relates to a method of performing a strand resynthesis extension reaction during paired sequencing, wherein the strand resynthesis extension reaction is performed between a first sequencing read and a second sequencing read, and wherein the strand resynthesis extension reaction extends one or more immobilized primers to copy a first template strand, thus, a second immobilized template chain is generated; the method is characterized in that the chain resynthesis extension reaction is carried out using a non-thermally stable chain displacement polymerase at a temperature of less than 55 DEG C, preferably 38 DEG C. 本发明涉及对高通量核酸测序方法的改进，并且特别是涉及对在成对测序期间进行延伸反应的方法的改进。本发明涉及一种在成对测序期间进行链再合成延伸反应的方法，其中所述链再合成延伸反应在第一测序读取与第二测序读取之间进行，并且其中所述链再合成延伸反应延伸一种或多种固定化引物以拷贝第一模板链，从而生成第二固定化模板链；其特征在于，所述链再合成延伸反应使用非热稳定链置