Kit for live pig transportation stress early warning and early warning method thereof

The invention is applicable to the technical field of in-vivo stress early warning, and provides a kit for live pig transportation stress early warning, which comprises a microwell plate, a pig DAO standard substance, a cleaning solution, a confining liquid, a phosphate buffer solution, a DAO polyclonal antibody, an HRP labeled secondary antibody, a phosphate buffer solution, a TMB substrate solution and a stop solution. The invention further provides a live pig transportation stress early warning method applying the kit. According to the correlation between the serum DAO content and live pig transportation stress early warning, whether a live pig stress reaction occurs or not can be accurately judged through ELISA detection of DAO in the blood of the live pig to be detected, early warning is provided for live pig transportation stress, and economic losses caused by transportation stress can be effectively reduced. 本发明适用于活体应激预警技术领域，提供了一种用于生猪运输应激预警的试剂盒，所述包括微孔板、猪DAO标准品、洗涤液、封闭液、磷酸盐缓冲液、DAO多克隆抗体、HRP标记的二抗、磷酸盐缓冲液、TM