RNA (Ribonucleic Acid) fluorescent probe as well as preparation method and application thereof
The invention relates to the technical field of probe detection, in particular to an RNA (Ribonucleic Acid) fluorescent probe as well as a preparation method and application thereof. The RNA fluorescent probe disclosed by the invention is a novel probe synthesized on the basis of a thiophene compoun...
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| creator | CHEN ZHE ZHANG YUE LI BINGYAN SHEN JIANPING WEI GUOLING LIU YONG |
| description | The invention relates to the technical field of probe detection, in particular to an RNA (Ribonucleic Acid) fluorescent probe as well as a preparation method and application thereof. The RNA fluorescent probe disclosed by the invention is a novel probe synthesized on the basis of a thiophene compound, and the probe can be combined with RNA through hydrogen bond interaction, intercalation and the like by virtue of the characteristic that a V-shaped conjugated structure and a rigid plane of the probe are connected in a single bond form. After RNA is embedded, free rotation of a single bond of the probe is limited to generate a twisted intramolecular charge transfer (TICT) mechanism effect, so that RNA can be biologically imaged through a fluorescence detection method, and particularly RNA detection in a cell or water environment can be realized. According to the record of the embodiment, the RNA fluorescent probe disclosed by the invention not only has high stability, low background fluorescence and high sensit |
| format | Patent |
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| subjects | ADHESIVES CHEMISTRY DYES HETEROCYCLIC COMPOUNDS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE MEASURING METALLURGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS NATURAL RESINS ORGANIC CHEMISTRY PAINTS PHYSICS POLISHES TESTING |
| title | RNA (Ribonucleic Acid) fluorescent probe as well as preparation method and application thereof |
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