RNA (Ribonucleic Acid) fluorescent probe as well as preparation method and application thereof

The invention relates to the technical field of probe detection, in particular to an RNA (Ribonucleic Acid) fluorescent probe as well as a preparation method and application thereof. The RNA fluorescent probe disclosed by the invention is a novel probe synthesized on the basis of a thiophene compound, and the probe can be combined with RNA through hydrogen bond interaction, intercalation and the like by virtue of the characteristic that a V-shaped conjugated structure and a rigid plane of the probe are connected in a single bond form. After RNA is embedded, free rotation of a single bond of the probe is limited to generate a twisted intramolecular charge transfer (TICT) mechanism effect, so that RNA can be biologically imaged through a fluorescence detection method, and particularly RNA detection in a cell or water environment can be realized. According to the record of the embodiment, the RNA fluorescent probe disclosed by the invention not only has high stability, low background fluorescence and high sensit