LABEL-FREE DETECTION OF MYCOBACTERIA USING SURFACE ENHANCED RAMAN SPECTROSCOPY

A method of identifying a mycobacterium using surface enhanced Raman spectroscopy (SERS), disclosed herein, comprises disposing a sample suspected to comprise a mycobacterium on a SERS-active substrate, detecting surface enhanced Raman signals corresponding to an alpha-mycolic acid, a methoxy-mycoli...

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Bibliographische Detailangaben
Hauptverfasser:	KHAN SAIF ABDUL KADIR, OLIVO MALINI, PERUMAL JAYAKUMAR, GOPAL RENUGA
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	ACYCLIC OR CARBOCYCLIC COMPOUNDS BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of identifying a mycobacterium using surface enhanced Raman spectroscopy (SERS), disclosed herein, comprises disposing a sample suspected to comprise a mycobacterium on a SERS-active substrate, detecting surface enhanced Raman signals corresponding to an alpha-mycolic acid, a methoxy-mycolic acid, and a keto-mycolic acid from the sample disposed on the SERS-active substrate, and determining one or more ratios of intensity of the surface enhanced Raman signals to identify the mycobacterium. Disclosed herein also includes a method of detecting a mycobacterial disease using surface enhanced Raman spectroscopy (SERS), the method comprising identifying a mycobacterium according to the method described above, and determining the mycobacterial disease based on the mycobacterium identified. 本文公开了一种采用表面增强拉曼光谱(SERS)鉴定分枝杆菌的方法，包括：将疑似包括分枝杆菌的样品配置在SERS活性基底上；检测对应于配置在SERS活性基底上的样品中的α分枝菌酸(alpha-mycolic acid)、甲氧基分枝菌酸(methoxy-mycolic acid)以及酮基分枝菌酸(keto-mycolic acid)的表面增强拉曼信号；以及测定表面增强拉曼信号的一个或多个强度比率，以鉴定分枝杆菌。本文还公开了包括一种采用表面增