Primer and probe composition and kit for detecting achondroplasia related FGFR3 gene variation sites

The invention discloses a primer and probe composition and a kit for detecting achondroplasia related FGFR3 gene variation sites. The related FGFR3 gene variation sites comprise a first site and a second site, wherein the first site is NM_000142.5: exon10: c.1138G > A: p.Gly380Arg, and the second...

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Hauptverfasser:	JIANG XIEYUAN, GOU MO, DENG WEI, WEI XING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a primer and probe composition and a kit for detecting achondroplasia related FGFR3 gene variation sites. The related FGFR3 gene variation sites comprise a first site and a second site, wherein the first site is NM_000142.5: exon10: c.1138G > A: p.Gly380Arg, and the second site is NM_000142.5: exon10: c.1138G > C: p.Gly380Arg; the nucleotide sequence of an upstream primer of the first site and the second site is as shown in SEQ ID NO.1, and the nucleotide sequence of a downstream primer of the first site and the second site is as shown in SEQ ID NO.2; the nucleotide sequence of a wild probe at the first site and a wild probe at the second site is shown as SEQ ID NO.3, and the nucleotide sequence of a mutation probe at the first site is shown as SEQ ID NO.4; and the nucleotide sequence of a mutation probe at the second site is as shown in SEQ ID NO.5. The primer and probe composition is high in accuracy, low in false positive rate, low in cost, free of high-end instruments, simple in re