Suspension domestication method for high-titer lentivirus production based on HEK293T clone screening

The invention discloses a suspension domestication method for high-titer lentivirus production based on HEK293T clone screening, which comprises the following steps of: S1, carrying out clone screening of HEK293T adherent cells suitable for lentivirus packaging: screening out a plurality of first cl...

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Bibliographische Detailangaben
Hauptverfasser:	MA HONG, SUI LILI, HU DICHAO, KONG LINGJIE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a suspension domestication method for high-titer lentivirus production based on HEK293T clone screening, which comprises the following steps of: S1, carrying out clone screening of HEK293T adherent cells suitable for lentivirus packaging: screening out a plurality of first clone cells with higher infection efficiency, carrying out enlarged culture and building a library; S2, carrying out rapid suspension domestication on the screened HEK293T adherent cells to obtain HEK293T cells which are 93-98% in cell viability, good in cell dispersity and free of obvious aggregation and are successfully subjected to suspension domestication; and S3, screening suspension HEK293T high-titer lentivirus packaging clone cells: screening a virus supernatant with high infection efficiency, and obtaining corresponding clone cells, namely the high-yield suspension HEK293T cell clone of the lentivirus. According to the suspension domestication method for high-titer lentivirus production based on HEK293T clon